Year : 2018

Volume : Volume 9

Issue : Issue 3s

Address for correspondence :
Dr. Arushi Rastogi.
arushirastogi@ymail.com

Abstract

Background:  Liquid dish washing soap (DWS) presents as a new substitute for deparaffinizing in hematoxylin and eosin (H and E) staining method. Objective of the study was to evaluate and compare the quality of xylene alcohol free (XAF) deparaffinized sections with liquid DWS to that of conventional H and E sections.  Materials and Methods: Study included sections from thirty paraffin embedded tissue blocks, each stained by conventional H and E and XAF H and E staining method. Scoring was done on the basis of five parameters- nuclear staining, cytoplasmic staining (adequate = score1, inadequate = score0), clarity, uniformity and crispness of staining (present = score1, absent = score0). Score >/= 2 was inadequate for diagnosis and 3-5 was adequate for diagnosis. Statistical analysis was done using Z test. Results: Liquid DWS sections scored better for crispness (76.6%) and clarity (86.6%). Nuclear staining (96.6%), cytoplasmic staining (100%) and uniformity of staining (100%) were similar in both sections. 96.6% H and E sections stained adequately for diagnosis when compared with 100% in liquid DWS sections. Conclusion: Liquid DWS is a safer, non toxic, alternative and effective substitute to xylene in routine H and E staining procedure.

Keywords: Deparaffinization, liquid dish washing soap, xylene alcohol free.