Year : 2018

Volume : Volume 9

Issue : Issue 3

Address for correspondence :
Dr. Nikita Agrawal.
dr.nikita.agrawal@gmail.com

Abstract

Background:  Root canal failure accounts on the persistence of micro-organism inside the canal. Among them E faecalis was found causative in 12-90% of cases and C albicans had been isolated from 30-45% cases. Objective of intracanal medicaments is to reduce bacterial load inside the root canal system.1  Aims: The aim of present in vitro study was to determine the minimal inhibitory concentration of novel medicaments like Nisin, Neem, Propolis, Platelet rich plasma used against E faecalis and C albicans.  Methods & Material: Ethanolic extract at five different concentration (100%, 50%, 25%, 12.5%, 6.25%) were prepared of Nisin, Neem, Propolis and platelet rich plasma (n=5). Five well were punched on each Mullar-Hintan agar plate and 20 µl each of the concentrations of extracts were poured in the well. Strains of E faecalis and C albicans were inoculated and incubated for 24-48 hours at 37oC for observation. The diameter of the zone of inhibition was recorded in millimeters, using an inhibition zone measuring scale. The plates were duplicated in all the experiments. Statistical analysis: The results were statistically analyzed using one-way analysis of variance (ANOVA).  Results: There was significant increase in the zone of inhibition when the concentration of medicaments increases. Conclusion: Under the limitations of present in vitro study, it can be advocated that Propolis and Nisin were effective against E faecalis while Neem was effective against C albicans. However further clinical trails are required to confirm the results of present study.  

Keywords: intracanal medicaments, minimal inhibitory concentration, microflora.