Year : 2018

Volume : Volume 8

Issue : Issue 3

Address for correspondence :
Dr. Manthapuri Suvarna.
ManthapuriSuvarna@gmail.com

Abstract

Background:  The emergent need in promoting usage of safe and environmentally friendly chemicals in clinical laboratories. Aim: To organize a dye extract from Rubia cordifolia (Manjistha) to stain the histological sections as a substitute for eosin in terms of low cost and simple obtaining natural material. Methodology: Tissue sections are stained with dye obtained from roots of Rubia cordifolia instead of eosin. Results: The cells in the epithelium and connective tissue were stained red in colour. The cellular morphology was preserved, and clarity of cellular details are moderate. Conclusion: Manjistha can be used as an alternative stain for eosin.

Keywords: Rubia cordifolia, Manjistha, Organic dye.