Year : 2016

Volume : Volume 6

Issue : Issue 2s

Address for correspondence :
Dr. Chinar Fating
chinarfating@rediffmail.com

Abstract

Aim:  Evaluation of serum proteins was done in oral submucous fibrosis (OSMF) patients of different clinical stages and healthy controls. Materials and Method: Serum samples of 40 diagnosed cases of OSMF without any other systemic disease and age and sex matched 40 normal controls were subjected to SDS-PAGE on a 10% resolving gel, followed by staining with Coomassie Brilliant Blue R-250. Protein fractions were analyzed using computer software “Alpha Digi Doc 1201”. Results: The OSMF group showed increased mean total protein i.e. 7.69 g/dl when compared with control group i.e. 7.52g/dl. The serum sample of all patients showed altered protein profile for protein fractions ranging in molecular weight 136kDa, 120kDa and 93kDa, when they were subjected to SDS-PAGE and gels were analyzed. The milder grades of OSMF showed overexpression for the above mentioned protein fractions and severe grade which is accompanied by increased duration, frequency and amount of betel nut consumption, these protein fractions were observed to be consistently absent or under expressed. Conclusion: The above result establishes the fact of alteration in serum protein profile in OSMF. The preliminary analysis of serum protein profile may act as diagnostic marker in oral Submucous fibrosis, a premalignant condition with a marked malignant potential. As our results specify that in severe grades of OSMF there is decreased or loss of particular protein fractions so this could also predict the prognosis and progression of the condition to malignancy with greater accuracy. Keywords: Oral submucous fibrosis, serum protein profile, SDS-PAGE.