Year : 2016

Volume : Volume 6

Issue : Issue 1

Abstract

Aim:  Hence the objective of this study is to evaluate the effectiveness of 2% chlorhexidine and 2% glutaraldehyde on the adherence of Candida albicans on heat cure acrylic resin, cobalt-chromium alloy and flexible denture base materials. Methodology: Total 90 samples were made, 30 from each type of denture base material i.e. Heat cure acrylic resin followed by flexible acrylic resin and cobalt chromium alloy. All disc samples were sterilized with ultraviolet rays. For Candida albicans adherence, all group samples were placed in standardized cell suspension (ATCC 26555) of Candida albicans for 1 hour at room temperature. After the samples were removed, drained and placed in 1ml phosphate buffer saline (PBS), and vortexed for 1h. A loop full of the content were transferred from phosphate buffer saline (PBS) on to Yeast Peptone Dextrose (YPD) Agar plate using 4mm diameter standard wire loop. This material were spread on the plate in a lawn culture and incubated at 37°c for 48 hr.  Viable counts of the microorganism were determined by colony count. Results were expressed in cfu/mm2. To test the antifungal efficacy of glutaraldehyde and chlorhexidine, the procedure were repeated for all the disc samples of the all group where in the disc samples were treated with both the disinfectants separately for 12 hrs and tested for Candidal adherence. Results: It showed, The mean of CFU/mm2  of C. albicans on heat cure acrylic resin, flexible acrylic resin and cobalt-chromium alloy were 99.83 cfu/mm2, 80.21 cfu/mm2 and 69.90 cfu/mm2 respectively. The mean of CFU/mm2  of C. albicans after treated with 2% chlorhexidine and 2% Glutaraldehyde were zero. The mean of CFU/mm2  of C. albicans after treated with 0.2% chlorhexidine on heat cure acrylic resin and flexible acrylic resin were 0.87 cfu/mm2 and 1.33 cfu/mm2 respectively. Conclusion: The high candidal retention was observed on Heat cure acrylic resin followed by flexible acrylic resin and cobalt chromium alloy. At 0.2% chlorhexidine and 0.2% Glutaraldehyde there were slight growth of c. albicans on Heat cure and flexible acrylic resin but no growth on Co-Cr alloy material. At 2% chlorhexidine and 2% Glutaraldehyde there were no growth of c. albicans on all three types of denture base material. Hence 2% hlorhexidine and 2% Glutaraldehyde can be used to soak the denture in denture wearing patients to avoid denture stomatitis.

Keywords: Denture Stomatitis, Denture biofilm, Chlorhexidine, glutaraldehyde, C.albicans, denture base material.