Journal of Research and Advancement in Dentistry
Official publication of Magadh University and Kolhan University, Government of Bihar and Jharkhand, India
Official publication of Magadh University and Kolhan University, Government of Bihar and Jharkhand, India
Year : 2024
Volume : Volume 16
Issue : Issue 2
Address for correspondence :
Dr. Vidhupriya B
Background: Candida-induced stomatitis is a significant concern for most medically compromised denturewearing patients. The combination of a tissue conditioner with an antifungal agent can effectively enhance the health of compromised tissues. This study aimed to evaluate and compare the antifungal efficacy of tissue conditioners incorporated with nanoparticles of graphene oxide, silver nanoparticles, and copper nanoparticles in inhibiting the growth of Candida albicans. Materials and Methods: Graphene oxide, silver nanoparticles, and copper nanoparticles were added at 5% by weight to the tissue conditioner material to form four groups: A, B, C, and D. Antifungal susceptibility was assessed using the well diffusion method with SDA on 90-mm plates. A total of five plates for each category were prepared and incubated at 37 °C for seven days. The tissue conditioner liquid and powder, each containing 5% GoNPs, 5% AgNPs, and 5% CuNPs respectively, were mixed and then filled into the punched-out wells. The diameter of the inhibition zone (DIZ) was measured with a metallic scale at the end of one day and seven days. The collected data were subjected to statistical analysis using one-way ANOVA, Tukey’s multiple post hoc test, and the dependent t-test. Results: The addition of nano-graphene oxide in the tissue conditioner showed a statistically significant increase in antifungal activity against Candida albicans. The acrylic-based tissue conditioner with GO NPs exhibited the highest antifungal activity, followed by the tissue conditioner with CuNPs, and the least with the tissue conditioner containing AgNPs, both after the 1st day and the 7th day. In contrast, the control group showed no signs of an inhibition zone on day 17. Conclusion: Antifungal activity is present. Thus, graphene oxide, silver, and copper nanoparticles can be used to reduce fungal growth and have the potential to treat candida-induced denture stomatitis through drug delivery via tissue conditioners.
