Year : 2018

Volume : Volume 9

Issue : Issue 3

Address for correspondence :
Dr. Ankush Katkade.
AnkushKatkade@gmail.com

Abstract

Background:  The microflora in failed endodontic treatment consist mainly of, Enterococcus faecalis, Candida albicans and Actinomyces concomitans. Evaluating a protocol for disinfection in canals without having a toxic effect on peripheral tissue is important. The aim of this in vitro study was to check the efficacy of Nd: YAG Laser and 5.25 % Sodium Hypochlorite with and without an Antifungal Agent.  Materials and Methods: Root canals of single rooted permanent teeth were enlarged using the Protaper nickel titanium rotary system up to size 40 at 1 mm from the apical foramen. The inoculation of canals of the experimental teeth were done with 0.5ml of freshly prepared suspension and stored. Then the viles were incubated at 91% humidity and 37 degree Celsius for 96 hours. After  24 hours the experimental teeth were replaced with immediately prepared suspension containing Candida albicans. At 48 hours samples were plated on 4% SD agar plate to check the growth of Candida albicans. The samples were then divided into four groups- Group I- irrigation with sodium hypocglorite, Group II-  Irrigation with sodium hypochlorite and antifungal agent, Group III- disinfection with LASER, Group IV- disinfection with LASER and antifungal agent. Conclusion-Under the limitations of a laboratory study of this kind, it can be concluded that Clotrimazole had a synergistic effect when used in conjunction with different disinfection protocols.

Keywords: Antifungal efficacy, 5.25% sodium hypochlorite, Candida albicans.