Year : 2016

Volume : Volume 6

Issue : Issue 1s

Abstract

Background:  Human oral cavity harbors a multitude of microorganisms. Among them Candida albicans has become a cause of great concern to dental profession.  Candida albicans has been widely associated with the etiology of denture related stomatitis. These lesions are very frequent complications in removable denture patients, especially the geriatric patients. A vast number of materials are used in the fabrication of denture bases, including acrylics, metals &more recently flexible denture base materials. Hence the objective of this study is to evaluate the adherence of Candida albicans on heat cure acrylic resin, cobalt-chromium alloy and flexible denture base materials. Methodology: Total 90 samples were made, 30 from each type of denture base material i.e. Heat cure acrylic resin followed by flexible acrylic resin and cobalt chromium alloy. All disc samples were sterilized with ultraviolet rays. For Candida albicans adherence, all group samples were placed in standardized cell suspension (ATCC 26555) of Candida albicans for 1 hour at room temperature. After the samples were removed, drained and placed in 1ml phosphate buffer saline (PBS), and vortexed for 1h. A loop full of the content were transferred from phosphate buffer saline (PBS) on to Yeast Peptone Dextrose (YPD) Agar plate using 4mm diameter standard wire loop. This material were spread on the plate in a lawn culture and incubated at 37°c for 48 hr.  Viable counts of the microorganism were determined by colony count. Results were expressed in cfu/mm2. Results: It showed The mean of CFU/mm2  of C. albicans on heat cure acrylic resin, flexible acrylic resin and cobalt-chromium alloy were 99.83 cfu/mm2, 80.21 cfu/mm2 and 69.90 cfu/mm2 respectively.  Interpretation & Conclusion: The high candidal retention was observed on Heat cure acrylic resin followed by flexible acrylic resin and cobalt chromium alloy.

Keywords: Denture Stomatitis, Denture biofilm, C.albicans, denture base material.