Year : 2019

Volume : Volume 10

Issue : Issue 2s

Address for correspondence :
Dr. Riddhi Vaghela.
riddhivaghela1993@gmail.com

Abstract

Background:  The purpose of this study is to evaluate the anti-microbial efficacy of two newer intracanal medicaments in the elimination of Enterococcus faecalis (e.faecalis) from the root canal system, a novel approach. Materials and Method: Thirty intact single-rooted permanent human teeth were collected for the study. Then samples were cleaned, and access cavities were prepared and the canals were instrumented with the rotary file system. During preparation, all the samples were irrigated with saline, CHX, sodium hypochlorite and EDTA, simultaneously. The apex of all teeth was sealed with the composite filling material. Teeth were autoclaved to ensure the absence of bacteria before bacterial inoculation. The root canals were then inoculated with the bacterial mixture and closed with the sterile sponge and temporary restoration. All samples were then incubated at 37°C for 48 hours of the growth of E.faecalis and its penetration into the dentinal tubules. All the samples were then divided into three groups of 10 each-Tigecycline, L-arginine and negative control group, respectively. Orifices closed with the sterile sponge and temporary restoration and incubated for 7 days. After that, all samples were irrigated with saline and with the help of H-files dentinal scraping were inoculated in nutrient broth. E.Faecalis colonies on agar plates were evaluated. Statistical analysis was done with One way ANOVA test and Post Hoc test. Result: Results showed almost negligible CFUs of e.faecalis in tigecycline compared to two other groups. There is a statistically significant difference between all three groups.  Conclusion: Tigecycline reduces bacterial growth significantly than two other groups.

Keywords: E. faecalis, Tigecycline, L- arginine.